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. 2018 Oct 2;100(2):514–522. doi: 10.1093/biolre/ioy207

Figure 2.

Figure 2.

Time course and concentration response of E2β on mRNA and protein expressions of CBS and CTH. Primary uterine artery endothelial cells (UAEC) were treated with 10 nM estradiol-17β (E2β) for up to 3 days to assess cystathionine β-synthase (CBS) and cystathionine γ-lyase (CTH) mRNA (A) and protein (B), or with increasing concentrations of E2β (0–1 μM) for 48 h to assess CBS and CTH mRNA (C) and protein (D). Data (means ± SEM) were collected from different cell preparations cells prepared from three to five ewes. Bars with different letters differ significantly (P < 0.05); capital and lower case letters pertain to CBS and CTH, respectively.