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. 2018 Oct 2;100(2):514–522. doi: 10.1093/biolre/ioy207

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© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

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Figure 3. — ER-dependency of E₂β effects on mRNA and protein expressions of CBS and CTH. Primary uterine artery endothelial cells (UAECs) were treated with vehicle, 10 nM estradiol-17β (E₂β), 1 μM ICI 182 780 (ICI), or both for 48 h. Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CTH) mRNA (A) and protein (B) were determined. (C) Immunofluorescence labeling of CBS and CTH proteins. Nuclei were stained with DAPI (blue). Protein expression was determined by relative green fluorescence intensity as fold change of control. Data (means ± SEM) were collected from different cell preparations from three to five ewes. *** P < 0.001 vs. Control. Scale bar = 50 μm.