Figure 2.

Male germ cell deletion of Rptor causes a progressive decline in spermatogenesis leading to infertility. (A–C) Representative images of unfixed whole testes from Rptor KO and WT littermates aged P8, P18, and adult P > 60 were acquired immediately post dissection. Note: there was a lighting issue on the day P8 testes were dissected (see A); actual colors were the same as in B nd C. (D–I) Hematoxylin and eosin-stained representative sections from Rptor WT (D–F) and KO (G–I) testes from P8, P18, and P > 60 animals. Yellow arrows in “D” point to condensed nuclei of preleptotene spermatocytes. (J) Average body weight, testis weight, seminal vesicle weight, and cauda epididymal sperm counts from adult (P > 60) Rptor WT and KO animals. Mean values are shown with standard deviation, and asterisks indicate statistical significance at P < 0.01. Scale bars = 50 μm.