Subject area	Biology
More specific subject area	Protein Synthesis, Translational Control, Ribosome, Bioinformatics, Transcriptomics, Translatomics
Type of data	Table and figures
How data was acquired	Ribosome profiling and RNA-Seq of wild-type or knockout yeast strains were performed. Sequences were obtained using Illumina HiSeq. 2000.
Data format	Raw and analyzed
Experimental factors	Saccharomyces cerevisiae BY4741 wild-type strain and BY4741-based strains with TMA20, TMA64 or both TMA20 and TMA64 knockouts were maintained in rich (YPD) or minimal (SD) media.
Experimental features	In the mid-log exponential phase, yeast cells were pretreated with cycloheximide and collected. cDNA libraries of ribosome-bound mRNA and total mRNA from wild-type and knockout strains were performed as described previously [1]. Sequenced reads were trimmed, read mapping and counting was performed.
Data source location	Moscow State University (Moscow, Russia)
Data accessibility	Analyzed data is presented in the article. Raw RNA-Seq and Ribo-Seq data as well as all gene counts are available in NCBI Gene Expression Omnibus (GEO) repository under GEO accession GSE122039 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE122039).
Related research article	N/A