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. 2019 Feb 16;5(2):e01231. doi: 10.1016/j.heliyon.2019.e01231

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Immunohistochemical staining of liver biopsy specimens from patients with viral hepatitis. (A) Liver biopsy specimens from patients with viral hepatitis (F0-3) were stained with Masson's trichrome and an antibody against R⁵⁸/LAP-DP (R⁵⁸) or α-SMA. The magnification images are derived from the black dashed squares. Insets show enlarged images of the representative areas. Tissue sections shown are representative of multiple biopsy samples, and the numbers in parentheses indicate the number of samples for each fibrosis stage. Scale bars = 100 μm. (B and C) Liver biopsy specimens from F2 and F3 patients were coimmunostained with antibodies against R⁵⁸/LAP-DP (red) and α-SMA (brown). Panels (B) and (C) are enlarged images of red dashed squares in F2 and F3 panels, respectively.