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SDS-PAGE analysis of purified mouse lipin 1β proteins. Mouse FLAG-tagged lipin 1β was expressed in HeLa cells and purified by affinity chromatography. Samples of the purified preparations of the WT (3.9 μg), S285A (1.7 μg), S287A (4.8 μg), and S285A/S287A (1.4 μg) lipin 1β were subjected to SDS-PAGE and stained with Coomassie Blue. The positions of lipin 1β and molecular mass standards (Std) are indicated.
