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Sequence analysis for Cn Cho1, Sc Cho1, and Ca Cho1. A, sequence alignment of Cho1 homologs from C. neoformans (Cn Cho1), S. cerevisiae (Sc Cho1), and C. albicans (Ca Cho1) using ClustalW. B, protein secondary structural prediction of Cn Cho1 based on HMMTOP2.0 suggests it has six transmembrane domains. The predicted enzyme activation sites are highlighted in blue.
