Figure 5.

Cn Cho1 is an essential protein for fungal viability. A Cn CHO1/cho1Δ heterozygous mutant was generated by deleting one Cn CHO1 allele in a diploid strain AI187 and undergoing sporulation on mating medium. Spores were isolated and grown on YPD or YPD + G418. Among the 28 spores isolated, none of them grew on YPD containing G418, indicating that they are all WT alleles. A, schematic of Cn CHO1 deletion strategy. CX numbers indicate primers used to screen the mutants. Detailed description of each primer can be found in Table S1. B, PCR results of the mutant screen. M1, mutant 1; M2, mutant 2. C, growth of progeny colonies on YPD and YPD containing G418 after 3 days of incubation.