Figure 6.
Inhibiting CHO1 expression leads to growth arrest independent of the Kennedy pathway. A, qRT-PCR analysis of PCTR4-CHO1 expression under different medium conditions. CHO1 gene expression was performed in triplicate, and two-tail t test was used for statistical analysis (* indicates p < 0.0001). B, growth rates of C. neoformans WT H99 and PCTR4-CHO1 cells on SC agar medium with indicated supplements. Addition of 1 mm ethanolamine or 1 mm choline did not rescue cell growth. C, growth of PCTR4-CHO1-expressing cells in SC liquid medium with indicated supplements. D, growth of H99 in SC medium in the presence or absence of 1 mm CuSO4. Each growth assay was done in triplicate and repeated at least twice.