Figure 2.

Regulation of MEF2D Ser-444 phosphorylation. A, C2C12 cells transfected with an expression plasmid for GFP- and FLAG-tagged MEF2D were cultured in GM or DM in the absence or presence of cyclosporin A (500 nm) for 3 h before immunoprecipitation using FLAG antibodies and Western blotting using MEF2D and phospho-Ser-444 (P-MEF2D) antibodies. p (one-way ANOVA) = 0.0002. B–D, same as in A except using cells co-transfected with mAKAP or control siRNA (B), mCherry or CBD-mCherry expression plasmids (C), or mCherry or MBD-mCherry expression plasmids. n = 3 independent experiments for all panels. p (two-way ANOVA for both factors and interaction) < 0.03 for B–D. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001. Error bars, S.E.