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. 2018 Dec 11;294(7):2500–2518. doi: 10.1074/jbc.RA118.004926

Figure 13.

Figure 13.

Cardiac pressure overload induces USP20 phosphorylation in a β1AR-dependent manner. A, soluble extracts from left ventricles of WT mice with sham or TAC surgery were immunoblotted for p-USP20 and total USP20. B, quantification of p-USP20 normalized to USP20, sham n = 5, and TAC n = 11, **, p = 0.003, unpaired, two-tailed t test. The maximum p-USP20/USP20 ratio in the experimental cohort was taken as 100%. C and D, soluble extracts from left ventricles of mice that underwent sham or TAC surgery of indicated genotypes were serially immunoblotted for p-USP20 and total USP20. E, graph shows quantification of p-USP20 normalized to USP20 plotted as in B. *, p < 0.05, ANOVA, Bonferroni's post-test.