Figure 9.
Cardiac morphology, histopathology, and morphometry of WT and USP20–KO mice. A, immunoblot of cardiac lysates from 3- to 4-month-old WT and USP20–KO mice with USP20 and GAPDH antibodies. Blots are representative of similar results obtained from >10 mice of each genotype. B, representative whole hearts from 3- to 4-month-old WT and USP20–KO mice (n = 3, scale bar = 1 mm). C, heart weight to body weight ratio from 3- to 4-month-old WT (n = 12) and USP20–KO (n = 12) mice. Error bars indicate average ± S.E. D, representative Masson Trichrome staining of cardiac sections from 3- to 4-month-old WT and USP20–KO (n = 5, scale bar, 0.5 mm). E, representative baseline echocardiograms via M-mode, short axis imaging, of WT and USP20–KO mice (see Table 1 for ECHO analyses).