Figure 4.

Serine 198 mutations do not alter MARCH9 subcellular localization. Representative images are shown for 293T cells expressing free cytosolic ZsGreen protein (empty vector, for reference) or MARCH9–ZsGreen fusion proteins with the indicated mutations. Cell preparations were stained and imaged by confocal microscopy 48 h after transient transfection. Color channels are ZsGreen (yellow), F-actin stain (Alexa 555-phalloidin, magenta), and nuclear stain (DAPI, cyan). All images contain both transfected and untransfected cells.