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. 2018 Dec 7;26(1):85–94. doi: 10.1093/dnares/dsy040

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© The Author(s) 2018. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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5 mC localization during spermatogenesis. DNA methylation was investigated by 5 mC immunohistochemical staining. DAPI and 5 mC stain nuclei blue and red, respectively (grey and white in black and white (BW) respectively). (A) Day 10 pp. Left-hand side: 5 mC staining (red); right-hand side SOX9 staining (green). (B) Day 25 pp. (C) Day 50 pp. (D) Day 70 pp. (E) Day 100 pp. (F) Day 200 pp. (G) and (H) Adult. All insets are negative controls. (A) Scale bar 8 μm. (B–F) Scale bars 20 μm. (G and H) Scale bars 50 μm. Abbreviations: Le, Leydig cells; PM, peritubular myoid cells; RS, Round spermatids; Se, Sertoli cells; SG, spermatogonia; SP, spermatocyte; Std, spermatid.