Table 1.
Target | Host | Product code | Concentration (mg/ml) | Dilution |
---|---|---|---|---|
Primary antibody | ||||
5-methylcytosine (5mC) | Mouse | ab10805 (Abcam) | 1 | 1/100 (Testes) |
1/50 (ovaries) | ||||
Human DNMT3L | Rabbit | ab3493 (Abcam) | 1.11 | 1/50 |
SOX9 | Rabbit | AB5535 (Merck) | 1 | 1/100 |
Secondary antibody | ||||
Mouse immunogen | Goat | Alexa Fluor 568 | 2 | 1/200 |
Rabbit immunogen | Donkey | Alexa Fluor 488 | 2 | 1/200 |