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Primers used for this study
| Primer | Sequence | Tm (°C) |
|---|---|---|
| For sequencing | ||
| Forward | 5ʹ-GATGGGTATCGTCCTCTTTG-3ʹ | 52.5 |
| Reverse | 5ʹ-CCTTAGGCTTTGCGTATTGT-3ʹ | 53.0 |
| For quantitative RT-PCR | ||
| DNMT3L | ||
| Forward | 5ʹ-ATGGACTACTGAAGAAGAGGAG-3ʹ | 53.2 |
| Reverse | 5ʹ-AGGGACAGAACTTGGATTGG-3ʹ | 54.6 |
| TBP | ||
| Forward | 5ʹ-GGACAAACTGAAGCAAAGGGACC-3ʹ | 58.5 |
| Reverse | 5ʹ-AGGGCATCATTGGGCTAAAGATAG-3ʹ | 56.8 |
| HMBS | ||
| Forward | 5ʹ-ACCTGACTGGAGGAGTATGGAGT-3ʹ | 58.8 |
| Reverse | 5ʹ-TGGGCTAAGATGTTGACGGTTGT-3ʹ | 58.7 |
