Table 1.

Equilibrium rates and barriers associated with Figure 6. Zero-load values for k_cleavage, k_ligation, and the rate limiting conformation changes associated with gate opening k_open, and closing, k_close. Δx_close values calculated from experiments and simulations are also shown. In the case of Topo III, this corresponds to Δx_close**, the distance between the fully open state and the barrier associated with formation of an open-state stabilizing salt bridge.

	kcleavage (s−1)	kligation (s−1)	kopen (s−1)	kclose (s−1)	Δxexp (nm)	Δxsim (nm)
Topo III	0.0012 ± 0.0004	1.5 ± 0.7	1.07 ± 0.17	1.8 ± 0.3	1.10 ± 0.04	1.3 ± 0.4
Topo I	0.9 ± 0.2	4.6 ± 1.0	4.1 ± 1.0	1642 ± 549	2.2 ± 0.1	2.9 ± 0.4