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. Author manuscript; available in PMC: 2019 Nov 21.
Published in final edited form as: Neuroscience. 2018 Sep 30;393:42–60. doi: 10.1016/j.neuroscience.2018.09.034

Figure 1.

Figure 1.

Schematic illustration representing the spinal cord slice procedure used to collect tissues for analysis. Following complete transection at cord level T8-T9, animals were allowed to recover from injury for a period of at least 28 days. Animals were sacrificed, the cord below the injury removed, and embedded for slicing. Longitudinal slices were made from the ventral surface of the cord and progressed in 300 μm increments. Slices of gray matter that contained concentrations of ChAT:GFP labeling were collected as motor neuron enriched slices (MNSlice). After removal of GFP containing neurons, the next most 300 μm dorsal slice was collected as interneuron enriched (INSlice). These slices were immediately placed in TriZol reagent and homogenized for total RNA extraction.