Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (D. melanogaster) |
Notch | NA | ID_FLYBASE:FBgn0004647 | |
| Gene (D. melanogaster) |
Mi-2 | NA | ID_FLYBASE:FBgn0262519 | |
| Gene (D. melanogaster) |
E(spl)mγ | NA | ID_FLYBASE:FBgn0002735 | |
| Gene (D. melanogaster) |
dpn | NA | ID_FLYBASE:FBgn0010109 | |
| Gene (D. melanogaster) |
zfh1 | NA | ID_FLYBASE:FBgn0004606 | |
| Genetic reagent (D. melanogaster) |
grh-Gal4 | PMID: 9651493 | Prokop et al., 1998 | |
| Genetic reagent (D. melanogaster) |
UAS-NΔecd | PMID: 8413612,8343959 | Fortini et al., 1993; Rebay et al., 1993 | |
| Genetic reagent (D. melanogaster) |
UAS-Mi-2-RNAi | Bloomington Stock Center | ID_BDSC:33419 | Genotype: y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00301}attP2 |
| Genetic reagent (D. melanogaster) | UAS-MTA1-like RNAi | Bloomington Stock Center | ID_BDSC:33745 | Genotype: y[1] sc[*] v[1]; P{y[+t7.7]v[+t1.8]=TRiP. HMS01084}attP2 |
| Genetic reagent (D. melanogaster) | UAS-Caf1p-55 RNAi | Bloomington Stock Center | ID_BDSC:34069 | Genotype: y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00051}attP2 |
| Genetic reagent (D. melanogaster) | UAS-zfh1-RNAi | Bloomington Stock Center | ID_BDSC:29347 | Genotype: y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02509}attP2/TM3, Sb[1] |
| Genetic reagent (D. melanogaster) | UAS-Pros RNAi | Bloomington Stock Center | ID_BDSC: 26745 | Genotype: y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02308}attP2/TM3, Sb[1] |
| Genetic reagent (D. melanogaster) |
UAS-zfh1-RB | Bloomington Stock Center | ID_BDSC: 6879 | made by Antonio Postigo (Siles et al., 2013); genotype: w[1118]; P{w[+mC]=UAS-zfh1.P}2B |
| Genetic reagent (D. melanogaster) |
H2Av-RFP | Bloomington Stock Center | ID_BDSC:23651 | w*; P {His2Av-mRFP1}II.2 |
| Genetic reagent (D. melanogaster) |
zfh1-GFP | PMID: 30002131 | Albert et al., 2018 | |
| Cell line (D. melanogaster) |
Kc 167 cells | Drosophila Genomics Resource Center | ID_DGRC: 1 | |
| Antibody | guinea pig polyclonal anti-Deadpan |
Christos Delidakis, Heraklion, Greece |
(Immuno fluorescence dilution 1:2000) |
|
| Antibody | rabbit polyclonal anti-Mi-2 | Alexander Brehm, Marburg, Germany |
(Immunofluorescence dilution 1:10,000) Kreher et al., 2017; PMID: 28378812 |
|
| Antibody | rabbit polyclonal anti-Asense |
Y.N.Yan, San Fransisco, USA |
(Immunofluorescence dilution 1:2000) Brand et al., 1993; PMID 8565817 |
|
| Antibody | mouse monoclonal anti-Pros |
Drosophila Hybridoma Studies Bank |
ID_DHSB: MR1A | (Immuno fluorescence dilution 1:50) |
| Antibody | mouse monoclonal anti-Mira |
Fumio Matsuzaki, Kobe, Japan | (Immuno fluorescence dilution 1:100) Ohshiro et al., 2000; PMID 11117747 |
|
| Antibody | goat polyclonal anti-Su(H) |
Santa Cruz Biotechnology |
ID_SC: sc15813 | (12 μl per ChIP with 15 × 106 cells) |
| Antibody | rabbit polyclonal anti-H3K56ac |
Active Motif | ID_Active Motif: 39281 | (3 μl per ChIP with 15 × 106 cells) |
| Antibody | rabbit polyclonal anti-H3K27me3 |
Millipore | ID_Millipore: 07–449 | (1 μl per ChIP with 15 × 106 cells) |
| Antibody | rabbit polyclonal anti-H3K27ac |
Abcam | ID_ABCAM: ab4729 | (10 μl per ChIP with 15 × 106 cells) |
| Antibody | rabbit polyclonal anti-H3 |
Abcam | ID_ABCAM: ab1791 | (1 μl per ChIP with 15 × 106 cells) |
| Sequence- based reagent |
This paper | oligonucleotides for mRNA levels and ChIP qPCR assays; Tables 1 and 2 | ||
| Commercial assay or kit |
MEGAscript T7 Transcription Kit |
ThermoFischer Scientific |
ID_TFS: AMB 13345 | |
| Commercial assay or kit |
Ambion,DNA-free kit | ThermoFischer Scientific |
ID_TFS: AM1906 | |
| Commercial assay or kit |
M-MLV reverse transcriptase |
Promega Corporation |
ID_Promega: M531A | |
| Commercial assay or kit |
LightCycler 480 SYBR Green I Master PCR Kit |
Roche | ID_Roche: 4707516001 | |
| Chemical compound, drug |
Collagenase | Sigma | ID_Sigma: C0130 |