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. 2019 Feb 18;8:e39598. doi: 10.7554/eLife.39598

Figure 6. ETV1 up-regulation is dependent on KLF2 and ERK.

Figure 6.

(a) Silencing of KLF2 in ASM-deficient fibroblasts results in reduced levels of ETV1, shown by a representative western blot of whole cell extracts, with quantification of band densities (mean ± s.e.m, n = 3) in adjacent plots. One way ANOVA p-values ***p<0.001. (b) ASM-deficient fibroblasts show increased ERK and mTORC1 activities, reduced AKT activity and unchanged AMPK activity, as shown by a representative western blot of whole cell extracts with band density quantification presented in the adjacent plot as mean ± s.e.m., n = 3. T-test p-values **p<0.01 ***p<0.001 (c) ERK inhibition by treatment with U0126 (20 µM, 16 hr) in ASM-deficient fibroblasts results in reduced ETV1 levels but does not affect KLF2, as shown by a representative western blot, with band density quantification in the adjacent plot depicted as mean ± s.e.m. for biological triplicates. T-test p-values **p<0.01 ***p<0.001.