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. Author manuscript; available in PMC: 2019 Feb 18.
Published in final edited form as: J Clin Lipidol. 2018 Oct 24;13(1):62–69. doi: 10.1016/j.jacl.2018.10.004

Figure. 2.

Figure. 2.

The plasma of the patient treated with IFN β1a contains immunoglobulins that bind to GPIHBP1 in a dose-dependent manner. uPAR-tagged human GPIHBP1 produced in Drosophila S2 cells was captured on an ELISA plate with the uPAR-specific monoclonal antibody R24. Serial dilutions of the patient plasma samples (pre-heparin and post-heparin samples) were applied to the wells and incubated overnight. Immunoglobulins bound to GPIHBP1 were detected with an HRP-labeled goat anti-human IgG/IgM. Plasma samples from a normal control and a subject with GPIHBP1 antibodies (AutoAb control)13 were included as negative and positive controls, respectively. HRP, horseradish peroxidase; GPIHBP1, glycosylphosphatidylinositol-anchored high-density lipoprotein–binding protein 1.