KEY RESOURCES TABLE
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-GFP chicken polyclonal antibody | Aves | Cat# GFP-1020; RRID: AB_10000240 |
Anti-rabies glycoprotein mouse monoclonal antibody | Millipore | Cat# MAB8727; RRID: AB_571110 |
Anti-tyrosine hydroxylase rabbit polyclonal antibody | Millipore | Cat# AB152; RRID: AB_390204 |
Anti-mCherry rat polyclonal antibody | Life Technologies (ThermoFisher) | Cat# M11217; RRID: AB_2536611 |
Anti-DIG sheep polyclonal antibody | Roche Applied Science (Sigma-Aldrich) | Cat# 1093274; RRID: AB_514496 |
Donkey anti-rabbit alexa 647 | Jackson ImmunoResearch | Cat# 711-605-152; RRID: AB_2492288 |
Donkey anti-rat Cy3 | Jackson ImmunoResearch | Cat# 712-165-153; RRID: AB_2340667 |
Donkey anti-chicken alexa 488 | Jackson Immunoresearch | Cat# 703-545-155; RRID: AB_2340375 |
Donkey anti-mouse alexa 488 | Jackson Immunoresearch | Cat# 703-545-155; RRID: AB_2340375 |
Bacterial and Virus Strains | ||
AAV5-CAG-FLExloxP-TC | UNC Vector Core; Beier et al., 2015 | N/A |
AAV8-CAG-FLExloxP-G | UNC Vector Core; Beier et al., 2015 | N/A |
AA V5-CAG-FLExFRT-TC | UNC Vector Core; Beier et al., 2015 | N/A |
AAV8-CAG-FLExFRT- G | UNC Vector Core; Beier et al., 2015 | N/A |
AA VDJ-hSyn1-FLExFRT-mGFP-2A-synaptophysin-mRuby | Stanford Viral Core; Beier et al., 2015 | N/A |
Chemicals, Peptides, and Recombinant Proteins | ||
Isoflurane | Henry Schein Animal Health | CAS# 26675-46-7; CHEBI:6015 |
DAPI | ThermoFisher Scientific | D1306 |
Pentobarbital | Vortech Pharmaceuticals | NDC 0298-9373-68 |
Tissue-plus O.C.T. Compound | ThermoFisher Scientific | Cat# 23-730-571 |
NeuroTrace blue | Invitrogen (ThermoFisher) | Cat#N21479 |
Experimental Models: Organisms/Strains | ||
Mouse: B6.SJL-SLc6a3tm1.1(cre)Bkmn/J (DAT-Cre) | The Jackson Laboratory | JAX:006660 |
Mouse: GAD2tm2(cre)zjh/J (GAD2-Cre) | The Jackson Laboratory | JAX: 010802 |
Mouse: Slc17a6tm2(cre)Lowl/J (vGluT2-Cre) | The Jackson Laboratory | JAX: 016963 |
Software and Algorithms | ||
ImageJ (Fiji) software | NIH | N/A |
MATLAB | MathWorks | https://www.mathworks.com/ |
GraphPad Prism | GraphPad | https://www.graphpad.com/ |
R; pvclust | The R Foundation | https://www.r-project.org/ |