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. 2019 Feb 12;13:32. doi: 10.3389/fncel.2019.00032

Figure 2.

Figure 2

Anatomical changes of dSPNs in the 6-OHDA lesion mouse model of PD. Spine density and dendritic arborization were measured in dSPNs in ex vivo slices obtained from control mice and compared to slices prepared from mice 30 (30 days lesion) or 60 (60 days lesion) days post 6-OHDA lesion of the medial forebrain bundle. (A) Sample two-photon images of distal dendritic segments illustrating reduced spine density at 60 days lesion compared to control but no change at 30 days lesion; scale bar denotes 10 μm. (B) Quantified spine densities from proximal and distal dendritic segments. dSPN spine density was decreased at 60 days post-lesion compared to control with no change at 30 days post lesion (proximal: control n = 22 neurons, 30 days lesion n = 20 neurons, and 60 days lesion n = 13 neurons; distal: control n = 21 neurons, 30 days lesion n = 20 neurons, and 60 days lesion n = 12 neurons). (C) Sample two-photon image of a dSPN from a control mouse. Concentric circles are spaced 10 μm apart and scale bar denotes 10 μm. (D) Sample two-photon images of dSPNs at 30 and 60 days post-lesion; dendritic arborization is reduced at both time points compared to control image presented in (C). (E) Sholl analysis of dSPNs from control (n = 22 neurons), 30 days lesion (n = 19 neurons) and 60 days lesion (n = 11 neurons); solid lines represent the mean and shaded lines the SEM. (F) Total dendritic length was reduced in slices obtained from 30 days lesion and 60 days lesion mice compared to controls (control n = 22 neurons, 30 days lesion n = 19 neurons, and 60 days lesion n = 11 neurons); *p < 0.05.