FIGURE 2.

Cholesterol depletion increases filopodium-like structures in hippocampal neurons and EGL neurons. Examples of hippocampal (A) and EGL (B) filopodium-like structures in axons treated with control culture media, 2.5 μg/ml Nystatin, 2U ChOx, and 0.5 mM MβCD and stained with phalloidin. The density of filopodium-like structures was measured in discrete segments of axons in three independent experiments. Data represent means ± SEM (C,D). N = 150–250 neurons in each condition (One-way ANOVA, Dunnett’s Multiple comparison test ^∗∗p < 0.01, ^∗∗∗p < 0.0001). Scale bar 5 μm.