FIGURE 6.

Nystatin increases EC axon regeneration in organotypic cultures. (A) Schematic diagram that summarizes the experimental protocol. Slice co-cultures from P0 actin-GFP and WT mice were isolated, as described in Section “Materials and Methods” and kept in culture 21 DIV. Axotomy of the EHP was performed, and slices were treated for 10 DIV with control medium or 5 μg/ml Nystatin. (B) Representative examples of control hippocampal slices show virtually no regenerative axons (upper panel) or very few regenerative axons (lower panel). (C) Examples of hippocampal slices treated with Nystatin show moderate axonal regeneration (upper panel) or robust axonal regeneration (lower panel). Axons that cross a 250 μm line were counted in three random fields near the dentate gyrus in each slice. Data represent means ± SEM (D). One dot corresponds to one slice (t-Test ^∗p < 0.05). Scale bar 100 μm. ML, molecular layer; GL, granule cell layer; H, hilus.