Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Simplified workflow for CRISPR/Cas9-mediated plant genome editing. The production of edited plants with a desired phenotype starts from the design of a sgRNA for a specific target sequence and cloning of the sequence to express the sgRNA into a binary vector containing the Cas DNA sequence. Then the delivery of CRISPR/Cas materials into the plant cell, followed by assays to confirm the presence of the edited events, and regeneration of whole plants.
