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. 2019 Feb 12;10:134. doi: 10.3389/fimmu.2019.00134

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Copyright © 2019 Fusade-Boyer, Dupré, Bessière, Khiar, Quentin-Froignant, Beck, Lecollinet, Rameix-Welti, Eléouët, Tangy, Lajoie, Bertagnoli, Vidalain, Gallardo and Volmer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the contribution of eIF2α phosphorylation to the antiviral effects of Sephin1. Cells were either left non-infected (NI) or infected with the indicated viruses, in the presence or absence of 50 μM Sephin1. Sodium arsenite (As), a potent inducer of eIF2α phosphorylation, was used as a positive control for detection of eIF2α phosphorylation by western-blot. (A) Analysis of eIF2α phosphorylation in HEp-2 cells infected with hRSV. (B) Analysis of eIF2α phosphorylation in HEK293T cells infected with measles virus. (C) Analysis of eIF2α phosphorylation in RK13 cells infected with myxoma virus. (D) Consequences of Sephin1 treatment on TMEV replication in WT mouse embryonic fibroblasts (MEF WT). (E) Consequences of Sephin1 treatment on TMEV replication in mouse embryonic fibroblasts expressing a non-phosphorylable (S51A) allele of eIF2α (MEF S51A). TMEV replication was determined by measuring mCherry fluorescence. Data represent mean ± SEM from representative experiments, repeated at least three times.