Figure 2.

Evaluation of the antiviral properties of Sephin1 against hRSV. (A) HEp-2 cells were infected with a recombinant strain of hRSV expressing luciferase and incubated with increasing doses of Sephin1 or DMSO alone. After 24 h, luciferase expression was determined. (B) Viability of HEp-2 cells incubated with increasing doses of Sephin1 or DMSO alone was determined after 24 h of incubation using the cellular viability assay Vita-Blue. (C) HEp-2 cells were infected with a recombinant strain of hRSV expressing mCherry and incubated with increasing doses of Sephin1 or DMSO alone. After 24 h, cells were fixed and imaged using a confocal microscope. Data represent mean ± SEM from representative experiments, repeated at least three times. ^*p < 0.05, ^**p < 0.01 by one-way analysis of variance, followed by Bonferroni comparison test comparing the Sephin1-treated group at the indicated concentration to the vehicle-treated control group.