Figure 10.

Effects of cytochalasin D, NSC237766, and MG132 on the BP IgG-induced decrease in the number of adherent cells. NHEKs were pretreated with cytochalasin D (20 μM) for 30min, NSC23766 (500 μM) for 1 h, or MG132 (12.5 μM) for 0 h, and then 2 mg/ml BP IgGs (BP-1, BP-2, and BP-3) or normal IgG were added to the cultured cells. Total numbers of adherent cells after the incubation with BP IgGs or normal IgG were counted every hour in a ×20 area (0.57 mm²) for up to 6 h. One-way ANOVA was used for the statistical analysis. *p-value < 0.05.