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. 2019 Feb 12;10:200. doi: 10.3389/fimmu.2019.00200

Figure 5.

Figure 5

Changes in the morphology of NHEKs stimulated with BP IgG. (A) TEM images of NHEKs stimulated with BP IgGs (BP-1, BP-2, and BP-3) or normal IgG for 2 h. Zoomed-out images labeled with the # symbol shows vacuolar structures containing myelin-like structures. Vacuolar structures are indicated with arrows. The intracellular distribution of lysosomes in NHEKs stimulated with BP IgGs (BP-1, BP-2, and BP-3) or normal IgG. After a 2 h incubation with IgGs, cells were incubated with 50 nM LysoTracker® Green DND-26 for 15 min in dark, and live cells were imaged using confocal microscopy. Arrows indicate vesicles surrounded by lysosomes. Scale bar 10 μm. Y-axis in the left panel: fluorescence intensity. (C) Quantification of the fluorescently stained dots in cells. The LysoTracker® Green DND-26-stained dots in the cytoplasmic region of individual cells were counted using the CellProfiler image analysis software. One-way ANOVA was used for the statistical analysis. *p-value < 0.05. (D) Measurement of the fluorescence intensity per cell. The fluorescence intensity of LysoTracker® Green DND-26-stained cells was measured using CellProfiler image analysis software. One-way ANOVA was used for the statistical analysis. *p-value < 0.05.