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. 2019 Feb 18;9:2195. doi: 10.1038/s41598-018-37489-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extraction summary and preliminary screening. (a) A simplified summary of the isolated DPSC lines and the initial project design to model DPSCs aging in vitro. (b) Preliminary screening of cell lines with cell surface markers, CD29 and CD146 using western blot shows differential expression of these markers. (c,d) Fluorescent cytometry of candidate cell lines (DPSC 29,44,43,45) demonstrates high percentage of mesenchymal markers; CD29+CD146+ cells (c) and CD44+Stro1+ cells (d). (e) A representative graph shows that the percentage of “double positive” were comparable with commercial DPSC and MSC cell lines from Lonza. (n = 3 per cell line). Graph error bars are the means ± standard error of the mean (SEM).