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. 2019 Feb 18;9:2221. doi: 10.1038/s41598-018-38371-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ELISA results for cytokine production by gpD::GP2 displaying vaccinated mice. Animals were vaccinated with three subcutaneous injections of 10⁸ PFU gpD::GP2 every two weeks. Negative controls included 10⁸ PFU of λF7 or TN buffer. Two weeks after the last booster, sera were taken from three mice for each group and the concentrations of cytokines were measured using IFN-γ and IL-4 ELISA assay kits. Data are shown as mean ± SEM. (n = 3). ****P < 0.0001; denotes significant difference compared to controls.