Table 4.
Clustering of NORs in sperm cells. Percentage (%) of sperm cells in which NORs (recognized as FISH signals) form one, two, three, four or five clusters.
Number of NORs clusters | 1 | 2 | ∑(1 + 2) | 3 | 4 | 5 |
---|---|---|---|---|---|---|
Carrier | % of sperm cells | |||||
R1 rob(13;22) | 34.4* a | 38.5 Hb | 72.9* | 23.0* ,A | 4.1* L | 0.0 |
Numer of sperm cells | 134/390 | 150/390 | 284/390 | 90/390 | 16/390 | 0.0 |
∑ of analyzed sperm cells = 390 ∑ of analyzed NORs signals = 720 |
||||||
R2 rob(13;15) | 29.6* | 43.2 H | 72.8* | 23.0* A | 4.2* L | 0.0 |
Numer of sperm cells | 118/398 | 172/398 | 290/398 | 91/398 | 17/398 | 0.0 |
∑ of analyzed sperm cells = 398 ∑ of analyzed NORs signals = 786 |
||||||
R3 rob(13;14) | 28.2* | 44.4 H | 72.6* | 23.1* A | 4.3* L | 0.0 |
Numer of sperm cells | 99/351 | 156/351 | 255/351 | 81/351 | 15/351 | 0.0 |
∑ of analyzed sperm cells = 351 ∑ of analyzed NORs signals = 699 |
||||||
Control | 16.6 | 41.4 H | 58.0 | 32.1 | 9.4 | 0.4 L |
Numer of sperm cells | 79/474 | 197/474 | 276/474 | 153/474 | 45/474 | 2/474 |
∑ of analyzed sperm cells = 474 ∑ of analyzed NORs signals = 1122 |
One-way ANOVA was used to compare results (p ≤ 0.01 was considered to be statistically significant).
*Values significantly higher than the Control value (p < 0.01).
aSignificantly different from the R2 and R3 results.
bSignificantly different from the R2 and R3 results.
HSignificantly higher than the remaining results.
LSignificantly lower than the remaining results.
ASignificantly different from the remaining results.