Table 6.
Radial localization of NORs in sperm cells nuclei. Percentage (%) of NORs (recognized as FISH signals) in radial areas (a), (c1), (c2 + c3), (p1 + p3), (p2 + p4) and (t) as defined in Fig. 1B and depicted in Fig. 2.
| Radial areas in sperm cell• | a | c1 | c2 + c3 | p1 + p3 | p2 + p4 | t |
|---|---|---|---|---|---|---|
| Carrier | % of NORs | |||||
| R1 rob(13;22) | 21.9* A | 12.4 B | 15.6* | 26.2 H | 17.9 C | 6.0 L |
| Number of NORs signals | 158/720 | 89/720 | 112/720 | 189/720 | 129/720 | 43/720 |
| ∑ of NORs signals = 720 | ||||||
| ∑ of sperm cells = 390 | ||||||
| R2 rob(13;15) | 13.2 1,D | 10.0 | 18.8 E | 38.2* 2,H | 13.8* 3 | 6.0 L |
| Number of NORs signals | 104/786 | 78/786 | 148/786 | 300/786 | 108/786 | 48/786 |
| ∑ of NORs signals = 786 | ||||||
| ∑ of sperm cells = 398 | ||||||
| R3 rob(13;14) | 18.0 F | 12.9 G | 16.7* | 29.2* H | 14.6 | 8.6 L |
| Number of NORs signals | 126/699 | 90/699 | 117/699 | 204/699 | 102/699 | 60/699 |
| ∑ of NORs signals = 699 | ||||||
| ∑ of sperm cells = 351 | ||||||
| Control | 16.1 I | 12.4 J | 21.9 K | 23.4 M | 18.0 | 8.2 L |
| Number of NORs signals | 181/1122 | 139/1122 | 246/1122 | 262/1122 | 201/1122 | 92/1122 |
| ∑ of NORs signals = 1122 | ||||||
| ∑ of sperm cells = 474 | ||||||
•Radial areas according to the scheme shown in Fig. 1B. Radial areas were labeled as follows: (c1) = central; (c2 + c3) = internal,(p1 + p3) = peripheral, near the apical area, (p2 + p4) peripheral, near the tail area, (a) = apical area and (t) = tail area. One-way ANOVA was used to compare results (p ≤ 0.01 was considered to be significantly different). *Values significantly different from Control value (p < 0.01).
HIn cases when the R1, R2 and R3 values in the (p1 + p3) area were highest.
LIn cases when the of R1, R2, R3 and Control values in the t area were highest.
AValue not different only from the (p2 + p4) value.
BValue not different only from the (c2 + c3) value.
CValue not different only from the (c2 + c3) value
DValues of (a), (c1) and (p2 + p4) were not different.
EValue of (c2 + c3) was different from the other areas.
FValue not different only from the (c2 + c3) and (p2 + p4) values.
GValue not different only from the (p2 + p4) values.
IValue not different only from the (c1) and (p2 + p4) values.
JValue not different only from the (a) value.
KValue not different only from the (p1 + p3) and (p2 + p4) values.
MValue not different only from the (c2 + c3) value.
aSignificantly different from the R1 and R3 values.
bSignificantly different from the R1 and R3 values.
cSignificantly different from the R1 value.