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. 2019 Feb 18;10(3):165. doi: 10.1038/s41419-019-1425-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative immunoblots of P2Y12 protein expression. Total cell lysates from the ipsilateral spinal cord were used, and GAPDH was used as an internal control. b Densitometric analysis (six rats per group) of P2Y12 proteins normalized to the loading control. c Representative immunofluorescence images of P2Y12 (green) staining in the spinal dorsal horn after SNL surgery. d Quantitative analysis of the number of P2Y12-immunoreactive cells per 400-μm visual field length per section per rat in the spinal dorsal horn after SNL surgery. Values are normalized to the sham group. n = 3 rats per group, with three sections per rat. Values are represented as mean ± SEM. *p < 0.05 vs. sham group, **p < 0.01 vs. sham group, ***p < 0.001 vs. sham group