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. 2019 Feb 18;10(3):165. doi: 10.1038/s41419-019-1425-4

Fig. 7. P2Y12−/− mice exhibited alleviated nociceptive behavior and less excitatory synaptic transmission in lamina II neurons of spinal cord slice after partial sciatic nerve ligation (PSNL) surgery.

Fig. 7

Paw withdrawal reflexes in response to a defined mechanical stimulus (a, b) and thermal stimulus (c, d) were measured in wild-type and P2Y12−/− mice before and after PSNL surgery in the ipsilateral (a, c) and contralateral (b, d) hind paw. Reduced mechanical allodynia and thermal hyperalgesia were recorded in the ipsilateral hind paw of P2Y12−/− mice after PSNL surgery compared to wild-type mice (n = 6 mice per group). e Representative traces of miniature excitatory postsynaptic current (mEPSC) from lamina II (substantia gelatinosa [SG] area) neurons based on whole-cell recording. Statistics on mEPSC frequency (f) and amplitude (g) in different treatment groups (n = 10 neurons per group). Cumulative curves of mEPSC frequency (h, bin size = 25 ms) and amplitudes (i, bin size = 1 pA). There was no obvious difference between the two sham groups in mEPSC frequency or amplitude, while both the surgery groups exhibited higher frequencies and larger amplitudes, with lower values in the P2Y12−/− group. j Representative traces of action potential recordings evoked by 1000 pA current injection lasting 2 ms from lamina II (SG area) neurons, and resting membrane potential was recorded using the I = 0 mode. Quantification of resting membrane potential (k) and action potential (l) (n = 10 neurons per group). **p < 0.01 vs. P2Y12+/+ sham group, ***p < 0.001 vs. P2Y12+/+ sham group; #p < 0.05 vs. P2Y12−/− sham group, ##p < 0.01 vs. P2Y12−/− sham group, ###p < 0.001 vs. P2Y12−/− sham group; &p < 0.05 vs. P2Y12+/+ PSNL group, &&p < 0.01 vs. P2Y12+/+ PSNL group, &&&p < 0.001 vs. P2Y12+/+ PSNL group