Fig. 4.
MB neuron class-specific defects of Dscam localization in SPT mutants. a–l Distribution of Dscam[TM1]::GFP (a–f) and Dscam[TM2]::GFP (g–l) in lacek05/ + ((a–c) and (g–i)) and lacek05/lace2 ((d–f) and (j–l)) genetic background expressed using class-specific Gal4 expression lines, R16A06-Gal4 (γ neurons), R26E01-Gal4 (α′/β′ neurons), R65G04-Gal4 (α/β neurons). While images a–l represent the axonal half of the MB, the corresponding somato-dendritic area is represented in right upper insets. The white dotted rectangle represents the Dscam distribution in the axons, zoomed in and shown in the right lower inset. White dotted line on the right lower inset represents the line along which the line intensity profile was generated from a single confocal plane. The length of the inset is represented by the dotted black rectangle on the intensity profile. γ neurons show no change in the somato-dendritic or axonal distribution of Dscam[TM1]::GFP and Dscam[TM2]::GFP (a, d, g, j, q). α′/β′ and α/β neurons show formation of somato-dendritic aggregates with Dscam[TM1]::GFP mislocalizing to the axonal compartments (e, f, q) and Dscam[TM2]::GFP showing a irregular distribution in the axons (k, l, q). n(a) = 32, n(b) = 48, n(c) = 40, n(d) = 38, n(e) = 46, n(f) = 34, n(g) = 24, n(h) = 32, n(i) = 34, n(j) = 22, n(k) = 34, n(l) = 38. Scale Bar: 25 μm. Green: GFP, Red: FasII, Blue: N-Cad. Arrowheads indicate somatic aggregates of Dscam. m–p Single plane confocal images of somato-dendritic area (in the genotypes indicated) co-stained with TOTO-3 antibody to label the nuclei showed that Dscam[TM1]::GFP (o) and Dscam[TM2]::GFP (p) aggregates (arrowheads) are formed in the cell body region with a corresponding decreased localization in the dendritic compartment (white dotted line) in SPT mutants. Green: GFP, Blue: TOTO-3. Scale Bar: 25 μm. q Percentage of MB showing somatic aggregates and axonal mislocalization of Dscam[TM1]::GFP and Dscam[TM2]::GFP in different MB neuronal classes