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. 2019 Feb 18;9:2190. doi: 10.1038/s41598-019-38558-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Disruption of 3D microvessel alignment. (Ai) Day 5 scaffold exposed to interstitial fluid flow (direction denoted by white arrow) with hCMEC/D3 cd44KD cells and microvessel alignment plot (ii). (Bi) Day 5 hydrogel exposed to interstitial fluid flow with 0.5 μM blebbistatin and microvessel alignment plot (ii). GFP hBVP (green), Phalloidin-Texas Red (red), and DAPI (blue). Scale bars, 100 μm. (C,i) Western blot of cd44 protein expression levels from day 1 after transfection to day 7 and (ii) bar graph showing cd44 protein expression levels as relative intensity (RQ) normalized to day 7 values. Coomassie blue was used to control for gel loading. Data are presented as mean ± s.e.m. *P < 0.05, ***P < 0.001; statistical significance was calculated using Welch Two Sample t-test. Alignment values (n = 30) are from single samples per condition.