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. 2019 Feb 18;9:2205. doi: 10.1038/s41598-018-38257-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) The bisecting k-means segmentation of 49 sagittal tissue sections from a ND2:SmoA1 transgenic mouse brain containing a non-metastasizing cerebellum tumor showing clear delineation of grey matter (blue), white matter (red), and the primary tumor (yellow). (b) Visualization of the k-means segmentation map in a reconstructed 3D volume as a transverse virtual section.