Table 1.
Differential metabolites identified using negative-ion mode 3D-MALDI-MSI with statistically-different abundances in metastasizing primary tumors compared to non-metastasizing primary tumors in SmoA1-GFP murine medulloblastoma based on individual AUC values.
| Measured m/z | Theo. m/z | Mass accuracy (ppm) | AUC value* | Assigned Metabolite† |
|---|---|---|---|---|
| Decreased in metastasizing tissue | ||||
| 673.4811 | 673.4814 | 0.4 | 0.65 | PA (16:0_18:1) |
| 723.4968 | 723.4968 | 0.05 | 0.69 | PA (18:0_20:4) |
| 726.5440 | 726.5443 | 0.4 | 0.61 | PE (P-18:1/18:1) |
| PE( O -18:2/18:1) | ||||
| 738.5078 | 738.5079 | 0.1 | 0.64 | PE (16:0_20:4) |
| 742.5390 | 742.5392 | 0.3 | 0.66 | PE (18:1/18:1) |
| 760.5131 | 760.5134 | 0.4 | 0.67 | PS (16:0_18:1) |
| Increased in metastasizing tissue | ||||
| 834.5284 | 834.5291 | 0.8 | 0.40 | PS (18:0_22:6) |
| 885.5489 | 885.5499 | 1.1 | 0.31 | PI (18:0_20:4) |
| 965.5155 | 965.5156 | 0.1 | 0.37 | PIP (18:0_20:4) |
| 1045.4812 | 1045.4820 | 0.8 | 0.36 | PIP2 (18:0_20:4) |
See SI Fig. 5 for additional statistical testing.
*Values ≥ 0.6 were suspected to be decreased in the metastasizing tumor and lower than ≤ 0.4, increased in metastasizing tumors.
†All metabolites in table were detected as their [M-H]− ionic species.