Fig. 4.

Ectopic expression of PDEB1 in the PDEB1 KO restores SoMo. a Northern blot analysis of RNA from WT, PDEB1 KO and two addback clones, AB1 and AB2, expressing an ectopic copy of PDEB1 that is integrated upstream of a procyclin locus and transcribed from the procyclin promoter⁴⁴. The addback version has a truncated 3′-untranslated region derived from the EP1 procyclin gene, hence the smaller size of the transcript compared with the endogenous copy. The probe used is described in Fig. 2. 18S rRNA serves as a loading control⁶⁶. The expression of the ectopic copy relative to the endogenous copy is 4.3-fold for AB1 and 4.2-fold for AB2. b Growth of WT, PDEB1 KO, AB1, and AB2 parasites was monitored over the course of 5 days in suspension culture as described in Fig. 2b. c MSD is measured for WT, PDEB1 KO, AB1, and AB2 cells from two biological replicates. Results are from n = 1919 tracks for WT, n = 1824 tracks for PDEB1 KO, n = 1876 tracks for AB1, and n = 1973 tracks for AB2 from a total of 34 videos for each. Dotted lines represent standard error of the mean (SEM). d Community lifts demonstrate addbacks AB1 (left) and AB2 (right) are SoMo-positive and express both GPEET (red) and EP (green)