Fig. 7.

Impaired cAMP signaling impacts the prevalence and topology of infection. a Proventriculi at day 14 post infection. Nuclei of host cells are stained with Hoechst dye. In mixed infections (middle panels), only the dsRed-tagged cells are visible. Supplementary Movie 4 shows merged DIC and red fluorescent channels for a mixed infection with WT + KO-dsRed. b Midguts of flies infected with dsRed-tagged trypanosomes and fed with FITC-WGA 24 h prior to dissection at day 14. Nuclei of host cells are stained with Hoechst dye. Images in a and b were captured using a TillPhotonics/FEI iMIC digital spinning disc microscope. Scale bars: 100 microns