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. 2019 Feb 12;10:180. doi: 10.3389/fmicb.2019.00180

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Copyright © 2019 Xie, Chen, Zhang, Yuan, Xie, Zhang, Zheng, Wang, Li and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interactions of the ESCRT machinery based on the yeast two-hybrid assay. (A) The interaction of pGBKT7-53/pGADT7-T and pGBKT7-Lam/pGADT7-T were used as the positive and negative control, respectively. Interactions within (B) and between (C) ESCRT subcomplexes. Yeast transformants carrying the indicated constructs were diluted into different concentrations and plated onto the selective plates supplemented with X-gal and without Leu/Trp/His/Ade for assaying the growth and β-galactosidase (LacZ) activities. (D) A model depicting the protein-protein interactions of ESCRT machinery. Lines indicate the interactions that we have showed in this study.