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Co-labeling of ARO/Fos neurons in caudal Vc. (A) Example of co-labeled ARO/Fos neurons after TMJ stimulation in an OvX rat. Fos-positive neurons appeared as dark-stained nuclei (∗), while dual labeled ARO/Fos neurons appeared as dark nuclei surrounded by a lighter cytoplasmic compartment (#). (B) Summary of the percentage of ARO-positive neurons co-labeled for Fos or GAD, the biosynthetic enzyme for GABA, in male and OvX rats. b = p < 0.01 versus male. Scale bar = 30 μm.
