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. 2019 Feb 18;9:2224. doi: 10.1038/s41598-019-38598-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of MWCNTs on IL-8 release and mRNA expression. (a) dHL-60 and udHL-60 cells were exposed to MWCNTs for 24 h and then IL-8 release into culture medium was determined using ELISA. (b) dHL-60 cells were exposed to MWCNTs for 24 h and then IL-8 mRNA expression levels were measured using real-time PCR. Each transcript level was normalized to corresponding β-actin value and is expressed as relative units to untreated control. (c) Effects of Cyto D (0.5 μM) on the release and mRNA expression levels of IL-8 in dHL-60 cells exposed to 100 μg/mL _highFe-CNT. Values are means ± SD (n = 3). **P < 0.01 (versus untreated control, Dunnett, ANOVA). Statistical comparisons between the two groups were carried out using the Student’s t-test.