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. 2019 Feb 18;9:2224. doi: 10.1038/s41598-019-38598-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of BAY 11–7082 on IL-8 release and [Ca²⁺]_i. dHL-60 cells were pretreated with 5 μM BAY 11–7082 for 30 min and then exposed to 100 μg/mL _highFe-CNT for 24 h. IL-8 release into culture medium was determined by ELISA. [Ca²⁺]_i in dHL-60 cells was determined using Fluo 4-AM and flow cytometry. Values are means ± SD (n = 3). Statistical comparisons between the two groups were carried out using the Student’s t-test.