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. 2019 Feb 18;9:2224. doi: 10.1038/s41598-019-38598-4

Figure 6.

Figure 6

Effect of MWCNTs on apoptosis in dHL-60 cells. (a) dHL-60 cells were treated with 100 μg/mL MWCNTs for 24 and 48 h. The cells were stained with FITC-Annexin V and EthD-III for 15 min and then analyzed by flow cytometry. In each panel, the lower left quadrant shows healthy cells (Annexin V−/EthD-III−). Lower right, upper right, and upper left quadrants show early apoptosis (Annexin V+/EthD-III−), late apoptosis (Annexin V+/EthD-III+), and necrosis (Annexin V−/EthD-III+), respectively. (b) Data are summarized and expressed as percentage of total cell number. Values are means ± SD (n = 3).