Figure 1. dPIP4K functions in modulating cell size in larval salivary glands.

(A) Graph representing average cell size measurement (μm³) as mean ± S.E.M. of salivary glands from wandering third instar larvae of AB1Gal4/+ and overexpression of AB1>dPIP4K^WT, n=5. No significant change was observed when analysed by unpaired t test. (B) Representative confocal images of salivary glands from the genotypes a. AB1Gal4/+ b. AB1>dPIP4K^WT c. AB1Gal4/+;dPIP4K²⁹ and d. AB1>dPIP4K^WT;dPIP4K²⁹ showing cell size changes quantified in the earlier graph. Cell body is marked green by Bodipy conjugated lipid dye, nucleus is marked by TOTO3 shown in red. Scale bar indicated at 50 μm. Images are contrast adjusted for representation. (C) Graph representing average cell size (μm³) measurements as mean ± S.E.M. on reconstitution of dPIP4K^WT in the background of dPIP4K²⁹ as compared with AB1Gal4/+;dPIP4K²⁹, n=8. *P-value <0.05. (D) Graph representing average cell size (μm³) measurements as mean ± S.E.M. on reconstitution of dPIP4KUT^KD (where ‘UT’ indicates untagged protein) in the background of dPIP4K²⁹ as compared with AB1Gal4/+; dPIP4K²⁹, n=8. *P-value <0.05, ***P-value <0.01. (E) Comparison of protein levels between dPIP4KUT^WT and dPIP4KUT^KD as compared with AB1Gal4/+;dPIP4K²⁹ control in larval salivary glands, as seen on a Western blot probed by dPIP4K antibody. dPIP4K::eGFP which migrates ∼25 kDa higher than the untagged protein represented separately. Actin was used as the loading control for all.