Table 1.
Primers used for myod1, myog, igf1, mtor, fbxo32, rpl13, ppiaa and gapdh mRNA amplification by qPCR.
| Gene | Accession code | Primer (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| myod1 | comp144727_c1_seq4 | F: GTTCGTCGTCTTCCTCTTGC R: ACCCGTGCTTTAACACCAAC |
191 |
| myog | comp137034_c4_seq6 | F: CAGACCAGAGGTTTTATGAA R: TAGATGTTGGGGATGGCTTG |
171 |
| igf1 | comp134662_c2_seq2 | F: ATTCAGCAAGCCAACAGGT R: CGCACAATACATCTCAAGTCG |
116 |
| mtor | comp141640_c0_seq15 | F: TTGGGAGAGACGTACTGC R: CACAGGACTGGTGTAGGAA |
145 |
| fbxo32 | comp145335_c1_seq5 | F: TCTTTGGTGCTCCCCTTGTG R: TAAAACCGAGGACGGCTGG |
231 |
| rpl13 | comp141862_c0_seq1 | F: ATCAACAGGAAAGTAGCCC R: AGGATGAGTTTGGAGCGGTA |
122 |
| ppiaa | comp145566_c0_seq1 | F: ATTGTGGTTCGTGAAGTCGC R: CCGCTGGGCAGAGTGATTAT |
168 |
| gapdh | comp140533_c0_seq1 | F: ACACACGACGACAAGACCAA R: GTCCCTCTCGCTGAAAACTG |
267 |
F, forward; R, reverse. Gene are as follow: myod1 (myogenic differentiation 1), myog (myogenin), igf1 (insulin like growth factor 1), mtor (mechanistic target of rapamycin), fbxo32 (f-box protein 32), rpl13 (ribosomal protein L13), ppiaa (peptidylprolyl isomerase Aa) and gapdh (glyceraldehyde-3-phosphate dehydrogenase).