Table 2.
Target genes and sequences of primers used for qPCR analysis.
| Target gene | Primer | Primer sequence (5′-3′) | Accession number/reference |
|---|---|---|---|
| Lysozyme C | Lyso C Fw Lyso C Rv |
CGATGATGGCACTCCAGGT CATGCTTTCAGTCCTTCGGC |
EF203085.1 |
| Lysozyme G | Lyso G Fw Lyso G Rv |
CAATGGCTTTGGCCTCATGC CACGTGGGAAACTTTGCTCTG |
KC934746.1 |
| TNF-α | TNF-α Fw TNF-α Rv |
GGCGGCTTGAAAGTAGTGGA TATGCAGAACGTCGTGGTCC |
FN543477.1 |
| IL-10 | IL-10 Fw IL-10 Rv |
GCTCAGTGCAGAAGAGTCGAC CCCGCTTGAGATCCTGAAATATA |
[49] |
| IL-1β | IL-1β Fw IL-1β Rv |
GTACCCCACAAAACATCGGC CAAGAGCAGTTTGGGCAAGG |
AM932525.1 |
| TLR-4 | TLR-4 Fw TLR-4 Rv |
CTAAGAAAGTGCTTGGGCTTCAT GGTTTGTGGCAATAATGGCTTTC |
KX218428.1 |
| β-actin | β-actin Fw β-actin Rv |
GACTTCGAGCAGGAGATGG CAAGAAGGATGGCTGGAACA |
[29] |